AKT2

Protein-coding gene in the species Homo sapiens
AKT2
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

1GZK, 1GZN, 1GZO, 1MRV, 1MRY, 1O6K, 1O6L, 1P6S, 2JDO, 2JDR, 2UW9, 2X39, 2XH5, 3D0E, 3E87, 3E88, 3E8D

Identifiers
AliasesAKT2, v-akt murine thymoma viral oncogene homolog 2, HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA, AKT serine/threonine kinase 2
External IDsOMIM: 164731; MGI: 104874; HomoloGene: 48773; GeneCards: AKT2; OMA:AKT2 - orthologs
Gene location (Human)
Chromosome 19 (human)
Chr.Chromosome 19 (human)[1]
Chromosome 19 (human)
Genomic location for AKT2
Genomic location for AKT2
Band19q13.2Start40,230,317 bp[1]
End40,285,536 bp[1]
Gene location (Mouse)
Chromosome 7 (mouse)
Chr.Chromosome 7 (mouse)[2]
Chromosome 7 (mouse)
Genomic location for AKT2
Genomic location for AKT2
Band7 A3|7 15.94 cMStart27,290,977 bp[2]
End27,340,251 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • right uterine tube

  • right hemisphere of cerebellum

  • right lobe of thyroid gland

  • left lobe of thyroid gland

  • left ovary

  • right ovary

  • body of uterus

  • muscle of thigh

  • canal of the cervix

  • gastrocnemius muscle
Top expressed in
  • muscle of thigh

  • choroid plexus of fourth ventricle

  • genital tubercle

  • lip

  • granulocyte

  • ascending aorta

  • aortic valve

  • tail of embryo

  • lactiferous gland

  • yolk sac
More reference expression data
BioGPS


More reference expression data
Gene ontology
Molecular function
  • transferase activity
  • nucleotide binding
  • protein kinase activity
  • kinase activity
  • protein serine/threonine kinase activity
  • protein binding
  • ATP binding
  • metal ion binding
Cellular component
  • cytoplasm
  • endosome
  • membrane
  • plasma membrane
  • nucleoplasm
  • ruffle membrane
  • cell cortex
  • early endosome
  • nucleus
  • cytosol
  • intracellular membrane-bounded organelle
  • protein-containing complex
  • intracellular anatomical structure
Biological process
  • positive regulation of protein targeting to membrane
  • apoptotic process
  • positive regulation of fatty acid beta-oxidation
  • insulin receptor signaling pathway
  • positive regulation of protein phosphorylation
  • intracellular signal transduction
  • regulation of cell migration
  • peripheral nervous system myelin maintenance
  • positive regulation of glycogen biosynthetic process
  • positive regulation of cell motility
  • intracellular protein transmembrane transport
  • activation of GTPase activity
  • phosphorylation
  • positive regulation of glucose metabolic process
  • mammary gland epithelial cell differentiation
  • multicellular organism development
  • protein phosphorylation
  • negative regulation of long-chain fatty acid import across plasma membrane
  • glycogen biosynthetic process
  • positive regulation of vesicle fusion
  • peptidyl-serine phosphorylation
  • glucose metabolic process
  • cellular response to insulin stimulus
  • glycogen metabolic process
  • protein localization to plasma membrane
  • regulation of translation
  • fat cell differentiation
  • signal transduction
  • carbohydrate transport
  • positive regulation of cell migration
  • positive regulation of glucose import
  • cellular response to high light intensity
  • retinal rod cell apoptotic process
  • positive regulation of cell population proliferation
  • positive regulation of mitochondrial membrane potential
  • negative regulation of apoptotic process
  • carbohydrate metabolic process
  • protein kinase B signaling
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

208

11652

Ensembl

ENSG00000105221

ENSMUSG00000004056

UniProt

P31751

Q60823

RefSeq (mRNA)

NM_001243027
NM_001243028
NM_001626
NM_001330511

NM_001110208
NM_007434
NM_001331108
NM_001331109

RefSeq (protein)

NP_001229956
NP_001229957
NP_001317440
NP_001617

NP_001103678
NP_001318037
NP_001318038
NP_031460

Location (UCSC)Chr 19: 40.23 – 40.29 MbChr 7: 27.29 – 27.34 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

AKT2, also known as RAC-beta serine/threonine-protein kinase,[5] is an enzyme that in humans is encoded by the AKT2 gene.[6] It influences metabolite storage as part of the insulin signal transduction pathway.[5]

Function

This gene is a putative oncogene encoding a protein belonging to the AKT subfamily of serine/threonine kinases that contain SH2-like (Src homology 2-like) domains. The encoded protein is a general protein kinase capable of phosphorylating several known proteins.[7]

AKT2 has important roles in controlling glycogenesis, gluconeogenesis, and glucose transport as part of the insulin signal transduction pathway.[5]

Clinical significance

The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.[7]

Mice lacking Akt2 have a normal body mass, but display a profound diabetic phenotype, indicating that Akt2 plays a key role in signal transduction downstream of the insulin receptor. Mice lacking Akt2 show worse outcome in breast cancer initiated by the large T antigen as well as the neu oncogene.[8]

Interactions

AKT2 has been shown to interact with:

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000105221 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000004056 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ a b c Tsoukas MA, Mantzoros CS (2016-01-01), Jameson JL, De Groot LJ, de Kretser DM, Giudice LC (eds.), "Chapter 37 - Lipodystrophy Syndromes", Endocrinology: Adult and Pediatric (Seventh Edition), Philadelphia: W.B. Saunders, pp. 648–661.e5, doi:10.1016/b978-0-323-18907-1.00037-8, ISBN 978-0-323-18907-1, retrieved 2020-12-18
  6. ^ Cheng JQ, Godwin AK, Bellacosa A, Taguchi T, Franke TF, Hamilton TC, Tsichlis PN, Testa JR (November 1992). "AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas". Proc Natl Acad Sci U S A. 89 (19): 9267–71. Bibcode:1992PNAS...89.9267C. doi:10.1073/pnas.89.19.9267. PMC 50107. PMID 1409633.
  7. ^ a b "Entrez Gene: AKT2 v-akt murine thymoma viral oncogene homolog 2".
  8. ^ Heron-Milhavet L, Khouya N, Fernandez A, Lamb NJ (2011). "Akt1 and Akt2: differentiating the aktion". Histol. Histopathol. 26 (5): 651–62. PMID 21432781.
  9. ^ Mitsuuchi Y, Johnson SW, Sonoda G, Tanno S, Golemis EA, Testa JR (September 1999). "Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2". Oncogene. 18 (35): 4891–8. doi:10.1038/sj.onc.1203080. PMID 10490823.
  10. ^ Yuan ZQ, Feldman RI, Sun M, Olashaw NE, Coppola D, Sussman GE, Shelley SA, Nicosia SV, Cheng JQ (August 2002). "Inhibition of JNK by cellular stress- and tumor necrosis factor alpha-induced AKT2 through activation of the NF kappa B pathway in human epithelial Cells". J. Biol. Chem. 277 (33): 29973–82. doi:10.1074/jbc.M203636200. PMID 12048203.
  11. ^ Figueroa C, Tarras S, Taylor J, Vojtek AB (November 2003). "Akt2 negatively regulates assembly of the POSH-MLK-JNK signaling complex". J. Biol. Chem. 278 (48): 47922–7. doi:10.1074/jbc.M307357200. PMID 14504284.
  12. ^ Laine J, Künstle G, Obata T, Noguchi M (February 2002). "Differential regulation of Akt kinase isoforms by the members of the TCL1 oncogene family". J. Biol. Chem. 277 (5): 3743–51. doi:10.1074/jbc.M107069200. PMID 11707444.
  13. ^ Laine J, Künstle G, Obata T, Sha M, Noguchi M (August 2000). "The protooncogene TCL1 is an Akt kinase coactivator". Mol. Cell. 6 (2): 395–407. doi:10.1016/S1097-2765(00)00039-3. PMID 10983986.

Further reading

  • Jones PF, Jakubowicz T, Hemmings BA (1992). "Molecular cloning of a second form of rac protein kinase". Cell Regul. 2 (12): 1001–9. doi:10.1091/mbc.2.12.1001. PMC 361900. PMID 1801921.
  • Staal SP (1987). "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma". Proc. Natl. Acad. Sci. U.S.A. 84 (14): 5034–7. Bibcode:1987PNAS...84.5034S. doi:10.1073/pnas.84.14.5034. PMC 305241. PMID 3037531.
  • Cheng JQ, Ruggeri B, Klein WM, Sonoda G, Altomare DA, Watson DK, Testa JR (1996). "Amplification of AKT2 in human pancreatic cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA". Proc. Natl. Acad. Sci. U.S.A. 93 (8): 3636–41. doi:10.1073/pnas.93.8.3636. PMC 39663. PMID 8622988.
  • Meier R, Alessi DR, Cron P, Andjelković M, Hemmings BA (1997). "Mitogenic activation, phosphorylation, and nuclear translocation of protein kinase Bbeta". J. Biol. Chem. 272 (48): 30491–7. doi:10.1074/jbc.272.48.30491. PMID 9374542.
  • Borgatti P, Zauli G, Colamussi ML, Gibellini D, Previati M, Cantley LL, Capitani S (1998). "Extracellular HIV-1 Tat protein activates phosphatidylinositol 3- and Akt/PKB kinases in CD4+ T lymphoblastoid Jurkat cells". Eur. J. Immunol. 27 (11): 2805–11. doi:10.1002/eji.1830271110. PMID 9394803. S2CID 6303471.
  • Walker KS, Deak M, Paterson A, Hudson K, Cohen P, Alessi DR (1998). "Activation of protein kinase B beta and gamma isoforms by insulin in vivo and by 3-phosphoinositide-dependent protein kinase-1 in vitro: comparison with protein kinase B alpha". Biochem. J. 331. ( Pt 1) (Pt 1): 299–308. doi:10.1042/bj3310299. PMC 1219352. PMID 9512493.
  • Joel PB, Smith J, Sturgill TW, Fisher TL, Blenis J, Lannigan DA (1998). "pp90rsk1 regulates estrogen receptor-mediated transcription through phosphorylation of Ser-167". Mol. Cell. Biol. 18 (4): 1978–84. doi:10.1128/mcb.18.4.1978. PMC 121427. PMID 9528769.
  • Delcommenne M, Tan C, Gray V, Rue L, Woodgett J, Dedhar S (1998). "Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase". Proc. Natl. Acad. Sci. U.S.A. 95 (19): 11211–6. Bibcode:1998PNAS...9511211D. doi:10.1073/pnas.95.19.11211. PMC 21621. PMID 9736715.
  • Mitsuuchi Y, Johnson SW, Sonoda G, Tanno S, Golemis EA, Testa JR (1999). "Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2". Oncogene. 18 (35): 4891–8. doi:10.1038/sj.onc.1203080. PMID 10490823.
  • Masure S, Haefner B, Wesselink JJ, Hoefnagel E, Mortier E, Verhasselt P, Tuytelaars A, Gordon R, Richardson A (1999). "Molecular cloning, expression and characterization of the human serine/threonine kinase Akt-3". Eur. J. Biochem. 265 (1): 353–60. doi:10.1046/j.1432-1327.1999.00774.x. PMID 10491192.
  • Meucci O, Fatatis A, Simen AA, Miller RJ (2000). "Expression of CX3CR1 chemokine receptors on neurons and their role in neuronal survival". Proc. Natl. Acad. Sci. U.S.A. 97 (14): 8075–80. Bibcode:2000PNAS...97.8075M. doi:10.1073/pnas.090017497. PMC 16672. PMID 10869418.
  • Laine J, Künstle G, Obata T, Sha M, Noguchi M (2000). "The protooncogene TCL1 is an Akt kinase coactivator". Mol. Cell. 6 (2): 395–407. doi:10.1016/S1097-2765(00)00039-3. PMID 10983986.
  • Campbell RA, Bhat-Nakshatri P, Patel NM, Constantinidou D, Ali S, Nakshatri H (2001). "Phosphatidylinositol 3-kinase/AKT-mediated activation of estrogen receptor alpha: a new model for anti-estrogen resistance". J. Biol. Chem. 276 (13): 9817–24. doi:10.1074/jbc.M010840200. hdl:1805/18872. PMID 11139588.
  • Zauli G, Milani D, Mirandola P, Mazzoni M, Secchiero P, Miscia S, Capitani S (2001). "HIV-1 Tat protein down-regulates CREB transcription factor expression in PC12 neuronal cells through a phosphatidylinositol 3-kinase/AKT/cyclic nucleoside phosphodiesterase pathway". FASEB J. 15 (2): 483–91. doi:10.1096/fj.00-0354com. PMID 11156964. S2CID 26315564.
  • Kapasi AA, Fan S, Singhal PC (2001). "Role of 14-3-3epsilon, c-Myc/Max, and Akt phosphorylation in HIV-1 gp 120-induced mesangial cell proliferation". Am. J. Physiol. Renal Physiol. 280 (2): F333–42. doi:10.1152/ajprenal.2001.280.2.F333. PMID 11208609. S2CID 29992656.
  • Matsumoto M, Ogawa W, Hino Y, Furukawa K, Ono Y, Takahashi M, Ohba M, Kuroki T, Kasuga M (2001). "Inhibition of insulin-induced activation of Akt by a kinase-deficient mutant of the epsilon isozyme of protein kinase C." J. Biol. Chem. 276 (17): 14400–6. doi:10.1074/jbc.M011093200. PMID 11278835.
  • Brownawell AM, Kops GJ, Macara IG, Burgering BM (2001). "Inhibition of nuclear import by protein kinase B (Akt) regulates the subcellular distribution and activity of the forkhead transcription factor AFX". Mol. Cell. Biol. 21 (10): 3534–46. doi:10.1128/MCB.21.10.3534-3546.2001. PMC 100275. PMID 11313479.
  • Clark DE, Poteet-Smith CE, Smith JA, Lannigan DA (2001). "Rsk2 allosterically activates estrogen receptor alpha by docking to the hormone-binding domain". EMBO J. 20 (13): 3484–94. doi:10.1093/emboj/20.13.3484. PMC 125527. PMID 11432835.
  • Chen R, Kim O, Yang J, Sato K, Eisenmann KM, McCarthy J, Chen H, Qiu Y (2001). "Regulation of Akt/PKB activation by tyrosine phosphorylation". J. Biol. Chem. 276 (34): 31858–62. doi:10.1074/jbc.C100271200. PMID 11445557.

External links

  • Human AKT2 genome location and AKT2 gene details page in the UCSC Genome Browser.
  • Overview of all the structural information available in the PDB for UniProt: P31751 (RAC-beta serine/threonine-protein kinase) at the PDBe-KB.
  • v
  • t
  • e
  • 1gzk: MOLECULAR MECHANISM FOR THE REGULATION OF PROTEIN KINASE B/ AKT BY HYDROPHOBIC MOTIF PHOSPHORYLATION
    1gzk: MOLECULAR MECHANISM FOR THE REGULATION OF PROTEIN KINASE B/ AKT BY HYDROPHOBIC MOTIF PHOSPHORYLATION
  • 1gzn: STRUCTURE OF PKB KINASE DOMAIN
    1gzn: STRUCTURE OF PKB KINASE DOMAIN
  • 1gzo: STRUCTURE OF PROTEIN KINASE B UNPHOSPHORYLATED
    1gzo: STRUCTURE OF PROTEIN KINASE B UNPHOSPHORYLATED
  • 1mrv: crystal structure of an inactive Akt2 kinase domain
    1mrv: crystal structure of an inactive Akt2 kinase domain
  • 1mry: crystal structure of an inactive akt2 kinase domain
    1mry: crystal structure of an inactive akt2 kinase domain
  • 1o6k: STRUCTURE OF ACTIVATED FORM OF PKB KINASE DOMAIN S474D WITH GSK3 PEPTIDE AND AMP-PNP
    1o6k: STRUCTURE OF ACTIVATED FORM OF PKB KINASE DOMAIN S474D WITH GSK3 PEPTIDE AND AMP-PNP
  • 1o6l: CRYSTAL STRUCTURE OF AN ACTIVATED AKT/PROTEIN KINASE B (PKB-PIF CHIMERA) TERNARY COMPLEX WITH AMP-PNP AND GSK3 PEPTIDE
    1o6l: CRYSTAL STRUCTURE OF AN ACTIVATED AKT/PROTEIN KINASE B (PKB-PIF CHIMERA) TERNARY COMPLEX WITH AMP-PNP AND GSK3 PEPTIDE
  • 1p6s: Solution Structure of the Pleckstrin Homology Domain of Human Protein Kinase B beta (Pkb/Akt)
    1p6s: Solution Structure of the Pleckstrin Homology Domain of Human Protein Kinase B beta (Pkb/Akt)
  • 2jdo: STRUCTURE OF PKB-BETA (AKT2) COMPLEXED WITH ISOQUINOLINE-5-SULFONIC ACID (2-(2-(4-CHLOROBENZYLOXY) ETHYLAMINO)ETHYL) AMIDE
    2jdo: STRUCTURE OF PKB-BETA (AKT2) COMPLEXED WITH ISOQUINOLINE-5-SULFONIC ACID (2-(2-(4-CHLOROBENZYLOXY) ETHYLAMINO)ETHYL) AMIDE
  • 2jdr: STRUCTURE OF PKB-BETA (AKT2) COMPLEXED WITH THE INHIBITOR A-443654
    2jdr: STRUCTURE OF PKB-BETA (AKT2) COMPLEXED WITH THE INHIBITOR A-443654
  • 2uw9: STRUCTURE OF PKB-BETA (AKT2) COMPLEXED WITH 4-(4-CHLORO-PHENYL)-4-(4-(1H-PYRAZOL-4-YL)-PHENYL)-PIPERIDINE
    2uw9: STRUCTURE OF PKB-BETA (AKT2) COMPLEXED WITH 4-(4-CHLORO-PHENYL)-4-(4-(1H-PYRAZOL-4-YL)-PHENYL)-PIPERIDINE
  • v
  • t
  • e
Non-specific serine/threonine protein kinases (EC 2.7.11.1)
Pyruvate dehydrogenase kinase (EC 2.7.11.2)
Dephospho-(reductase kinase) kinase (EC 2.7.11.3)
3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring) kinase (EC 2.7.11.4)
(isocitrate dehydrogenase (NADP+)) kinase (EC 2.7.11.5)
(tyrosine 3-monooxygenase) kinase (EC 2.7.11.6)
Myosin-heavy-chain kinase (EC 2.7.11.7)
Fas-activated serine/threonine kinase (EC 2.7.11.8)
Goodpasture-antigen-binding protein kinase (EC 2.7.11.9)
  • -
IκB kinase (EC 2.7.11.10)
cAMP-dependent protein kinase (EC 2.7.11.11)
cGMP-dependent protein kinase (EC 2.7.11.12)
Protein kinase C (EC 2.7.11.13)
Rhodopsin kinase (EC 2.7.11.14)
Beta adrenergic receptor kinase (EC 2.7.11.15)
G-protein coupled receptor kinases (EC 2.7.11.16)
Ca2+/calmodulin-dependent (EC 2.7.11.17)
Myosin light-chain kinase (EC 2.7.11.18)
Phosphorylase kinase (EC 2.7.11.19)
Elongation factor 2 kinase (EC 2.7.11.20)
Polo kinase (EC 2.7.11.21)
Serine/threonine-specific protein kinases (EC 2.7.11.21-EC 2.7.11.30)
Polo kinase (EC 2.7.11.21)
Cyclin-dependent kinase (EC 2.7.11.22)
(RNA-polymerase)-subunit kinase (EC 2.7.11.23)
Mitogen-activated protein kinase (EC 2.7.11.24)
MAP3K (EC 2.7.11.25)
Tau-protein kinase (EC 2.7.11.26)
(acetyl-CoA carboxylase) kinase (EC 2.7.11.27)
  • -
Tropomyosin kinase (EC 2.7.11.28)
  • -
Low-density-lipoprotein receptor kinase (EC 2.7.11.29)
  • -
Receptor protein serine/threonine kinase (EC 2.7.11.30)
MAP2K
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